RESEARCH PRODUCTS

2-Cys Peroxiredoxin Activity Assay Kit Quantitative Assay, suitable for detection of Prx I, II, III, IV, V via Spectrophotometric UV/Vis at 340nm, sufficient for 100 reactions, ultra accurate, ready-to-use, quick protocol, hyper sensitive, high reproducibility

Ordering information PRODUCT CODE PR-5011K UNIT PRICE $ 1,698.00 ($16.98 / Assays) AVAILABILITY In Stock  Ready-to-ship On Dec 18, 2018 FULFILLMENT BY LABSCOOP - SHIPS FROM LITTLE ROCK, AR 72201
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Overview

Peroxiredoxins (Prx) are a family of peroxidase proteins that reduce peroxides in the cells. In addition to reduction of peroxides these proteins are also implicated in many cellular functions such as differentiation, proliferation, and apoptosis. There are six different members of the mammalian peroxiredoxin family which are grouped as 2-Cys or 1-Cys peroxiredoxins. Peroxiredoxin isoenzymes I-V depend on reduced Thioredoxin for their reducing action. These are known as 2-Cys peroxiredoxins. Prx I is known to be involved in tumor suppression as an inhibitor of tyrosine kinase, and has been shown to be overexpressed in lung cancer. The overexpression of Prx I, II, V and VI has been shown to be increased in breast cancer. The levels of Prx I and Prx II has been shown to be significantly increased in Alzheimer’s disease. Recent studies have shown that mammalian peroxiredoxins are inactivated by oxidation of active site cysteine residues. In addition, recent studies have indicated that this oxidation process is reversible. Therefore, determination of activity of Prx is crucial in attributing biological function of Prx in conditions of oxidative stress in cancer, neurodegenerative diseases and in other diseases.

Mammalian 2-Cys peroxiredoxins are cellular peroxidases that reduce peroxides in the presence of Thioredoxin. Thioredoxin is oxidized in the process and is regenerated by Thioredoxin reductase that utilizes reducing equivalents from NADPH. In our assay method E.coli Trx and mammalian Thioredoxin reductase (TrxR) are used to provide reducing power to 2-Cys-Peroxiredoxins for reduction of peroxides such as hydrogen peroxide. The utilization of NADPH for the reduction of peroxides is monitored as the decrease in absorbance of NADPH at 340 nm. Specific activity is the amount of NADPH oxidized per minute per milligram of protein. A reaction blank is utilized in the absence of Thioredoxin to determine the non-specific decrease in the level of NADPH by mammalian TrxR.

Redoxica's 2-Cys-Peroxiredoxin Activity Assay Kit has undergone extensive testing with a multitude of lysate types, has been published in dozens of leading scientific journals and after a decade since its market debut has been established as the undisputed leader in the detection and activity measurement of 2-cys-peroxiredoxin. We developed the assay by utilizing core principles of biochemistry to ensure optimal accuracy, sensitivity, and reproducibility accompanied by a quick and easy to use protocol.

Specifications

Unit of Measure Assays
Shelf Life 24 MONTHS
Made In United States of America
Components • Peroxiredoxin Assay Buffer (2 x 50 mL bottles, Store at 2-8°C)
• Thioredoxin (2 x 250 μL vial, Store at -20°C)
• Thioredoxin Reductase (2 x 150 μL vial, Store at -20°C)
• β-NADPH (2 vials, Store at: -20°C, use within 3-4 hours of reconstitution, keep reconstituted NADPH on ice, not recommended for storage)
• Substrate Solution (1mL vial, Store at 2-8°C, light sensitive)
Required Materials Not Included • Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range.
• Quartz micro cuvettes capable of reading 50-300 µl volume in a 1-cm light path. Make sure that you use the right kind of micro-quartz cuvette for a specific spectrophotometer as they may vary.
Format Micro-cuvette
Synonyms Peroxiredoxin Activity Assay Kit, 2-Cys-Peroxiredoxin Activity Assay Kit, Peroxiredoxin 1 Assay Kit, Peroxiredoxin I Assay Kit, Prx 1 Assay Kit, Prx I Assay Kit, Peroxiredoxin 2 Assay Kit, Peroxiredoxin II Assay Kit, Prx 2 Assay Kit, Prx II Assay Kit, Peroxiredoxin 3 Assay Kit, Peroxiredoxin III Assay Kit, Prx 3 Assay Kit, Prx III Assay Kit, Peroxiredoxin 4 Assay Kit, Peroxiredoxin IV Assay Kit, Prx 4 Assay Kit, Prx IV Assay Kit, Peroxiredoxin 5 Assay Kit, Peroxiredoxin V Assay Kit, Prx 5 Assay Kit, Prx V Assay Kit, Peroxiredoxin Assay Kit, Prx Assay Kit, PRDX1 Assay Kit, PRDX2 Assay Kit, PRDX3 Assay Kit, PRDX4 Assay Kit, PRDX5 Assay Kit, PRDX Assay Kit, 2-Cys Prx Assay Kit, 2-Cys PRDX Assay Kit

Faq

Q. How much is shipping? A. Free shipping for US customers. If you are located outside of the United States, shipping costs can range between $30 - $250 USD excluding customs/duties/taxes/VAT.

Q. How do I get a lot specific certificate of analysis? A. The latest batch COA is available on the product page of our website. For a prior lot, please submit a request by visiting our "Contact Us" page. Be sure to provide the product code and batch number.

Q. Is there an MSDS available for this product? A. Yes, MSDS is available upon request.

Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request from the product detail page (directly below the order button). You can also submit a quote request from our contact us page.

Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop head quartered in the United States. You can place an order with them by clicking on the "Order on Labscoop" button on the product page or by submitting your purchase order by email to orders@labscoop.com, by fax at 1.800.316.3081 or by phone at 1.800.316.3081. You can also place an order with us directly by submitting an "Order Request" from our "Contact Us" page.

Q. Do you offer any discounts? A. We conduct an extensive price analysis on each product we release, so you can rest assured that you are getting a great price when comparing the quality of our products, the quantity, and free shipping and handling for our US customers. For bulk orders (quantities over 5), please submit a bulk quote request and in most cases we will be able to offer some discounts. For quantities under 5, unfortunately we do not offer any additional discounts.

Q. I still have questions, how do I contact technical support? A. No problem! Email us directly at support@redoxica.com

Product Citaions

  • Journal of Neuroscience

    Hu, X., Weng, Z., Chu, C. T., Zhang, L., Cao, G., Gao, Y., … Chen, J. (2011). Peroxiredoxin-2 protects against 6-hydroxydopamine-induced dopaminergic neurodegeneration via attenuation of the ASK1 signaling cascade. The Journal of Neuroscience : The Official Journal of the Society for Neuroscience, 31(1), 247–261. http://doi.org/10.1523/JNEUROSCI.4589-10.2011

    view abstract - Pmid: 21209210
  • Cancer Research

    Fiskus, W., Coothankandaswamy, V., Chen, J., Ma, H., Ha, K., Saenz, D. T., … Bhalla, K. N. (2016). SIRT2 deacetylates and inhibits the peroxidase activity of peroxiredoxin-1 to sensitize breast cancer cells to oxidant stress inducing agents. Cancer Research, 76(18), 5467–5478. http://doi.org/10.1158/0008-5472.CAN-16-0126

    view abstract - Pmid: 27503926
  • Neuro-Oncology

    Kim, T. H., Song, J., Kim, S.-H., Parikh, A. K., Mo, X., Palanichamy, K., … Kwon, C.-H. (2014). Piperlongumine treatment inactivates peroxiredoxin 4, exacerbates endoplasmic reticulum stress, and preferentially kills high-grade glioma cells. Neuro-Oncology, 16(10), 1354–1364. http://doi.org/10.1093/neuonc/nou088

    view abstract - Pmid: 24879047
  • Biotechnology and Bioengineering

    Chow‐shi‐yée, M. , Grondin, M. , Averill‐Bates, D. A. and Ouellet, F. (2016), Plant protein 2‐Cys peroxiredoxin TaBAS1 alleviates oxidative and nitrosative stresses incurred during cryopreservation of mammalian cells. Biotechnol. Bioeng., 113: 1511-1521. doi:10.1002/bit.25921

    view abstract - Pmid: 26724792
  • Biochimica et Biophysica Acta

    Myung-Chul Chung, Farhang Alem, Sarah G. Hamer, Aarthi Narayanan, Konstantin Shatalin, Charles Bailey, Evgeny Nudler, Ramin M. Hakami, S-nitrosylation of peroxiredoxin 1 contributes to viability of lung epithelial cells during Bacillus anthracis infection, Biochimica et Biophysica Acta (BBA), Volume 1861, Issue 1, Part A, 2017, Pages 3019-3029, ISSN 0304-4165, https://doi.org/10.1016/j.bbagen.2016.09.006. (http://www.sciencedirect.com/science/article/pii/S0304416516303282)

    view abstract - Pmid: 27612662
  • Journal of Cellular Biochemistry

    Lin, H. D., Fong, C. , Biswas, A. , Choolani, M. and Bongso, A. (2016), Human Umbilical Cord Wharton's Jelly Stem Cell Conditioned Medium Induces Tumoricidal Effects on Lymphoma Cells Through Hydrogen Peroxide Mediation. J. Cell. Biochem., 117: 2045-2055. doi:10.1002/jcb.25501

    view abstract - Pmid: 27392313
  • Cell Cycle

    Chu, K. L., Lew, Q. J., Rajasegaran, V., Kung, J. T., Zheng, L., Yang, Q., … Chao, S.-H. (2013). Regulation of PRDX1 peroxidase activity by Pin1. Cell Cycle, 12(6), 944–952. http://doi.org/10.4161/cc.23916

    view abstract - Pmid: 23421996
  • Antioxidants & Redox Signaling

    Gan, Y., Ji, X., Hu, X., Luo, Y., Zhang, L., Li, P., … Chen, J. (2012). Transgenic Overexpression of Peroxiredoxin-2 Attenuates Ischemic Neuronal Injury Via Suppression of a Redox-Sensitive Pro-Death Signaling Pathway. Antioxidants & Redox Signaling, 17(5), 719–732. http://doi.org/10.1089/ars.2011.4298

    view abstract - Pmid: 22356734

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STORAGE & SAFETY

Storage Temperature

Recommended to store assay buffer and substrate solution (LIGHT SENSITIVE) at 2-8°C (KEEP REFRIGERATED) and all other kit components at - 20°C (KEEP FROZEN, DRY). For lyophilized kit component storage upon reconsistution please refer to the product insert

Precautions & Disclaimer

For Laboratory Research and Development Use Only. Not for drug, household or other uses.

SHIPPING & HANDLING

Shipping Conditions

Dry Ice

Hazmat

No

Packaging

Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.

Shipping Weight

6.00 LBS

Shipping Dimensions

12"" X 12"" X 10""

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