Quantitative 1-mL UV/Vis spectrophotometric assay detection method, kit sufficient for 100 assays, ready-to-use, quick protocol, ultra high accuracy, hypersensitive, reliable, and consistent
The citric acid cycle enzyme aconitase is a member of a growing family of superoxide anion (O2.-) sensitive [4Fe—4S]-containing (de)- hydratases that has been implicated to be important sites of superoxide anion toxicity. The activity of aconitase is sensitive to inactivation by level of O2.- and is modulated by changes in O2.- levels in bacteria and mammalian cells. Thus, inactivation of aconitase can occur in response to oxygen radical generating compounds such as redox cycling agents, higher levels of oxygen or pathological conditions resulting in higher levels of O2.-. The assay is based on conversion of citrate to isocitrate by aconitase, and conversion of isocitrate to α-ketoglutarate by isocitric dehydrogenase. In this process, NADP+ is converted to NADPH, the absorbance of which is measured at 340 nm. The activity of aconitase is calculated as the rate of formation of NADPH and is expressed as milliunits of aconitase/mg protein.
Redoxica's Aconitase Assay Kit has undergone extensive testing with a number of lysate types, has been published in leading scientific journals and after a decade since its market debut has been recognized as an industry leader in Aconitase assaying technologies and other mitochondrial enzyme assay technologies. We developed the assay by utilizing core principles of biochemistry to ensure optimal accuracy, sensitivity, and reproducibility accompanied by a quick and easy-to-use protocol.
|Unit of Measure||Assays|
|Shelf Life||24 MONTHS|
|Made In||United States of America|
|Components||• Aconitase Assay Buffer (2 x 50 mL bottles, Store at 2-8°C)
• Aconitase Lysis Buffer (50 mL bottle, Store at 2-8°C)
• NADP (2 vials, Store at -20°C)
• Isocitric Dehydrogenase (1 vial, Store at: 2-8°C)
|Required Materials Not Included||• Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range with temperature control.
• Quartz semimicro cuvettes capable of holding 1-mL volume.
• Pipettors for accurately measuring various solutions and reagents.
• Dulbecco’s phosphate buffered saline (D-PBS) (Redoxica Product Code: PB9005-500ML)
|Synonyms||Aconitase Assay Kit, Aconitase Activity Assay Kit, Aconitate Hydratase Assay Kit|
Q. How much is shipping and handling? A. This product is temperature sensitive and does ship on dry ice. For US customers, S&H charges are $95. For international customers, shipping and handling costs will vary based on location and order size. Additional customs/duties/VAT charges may be incurred for international orders.
Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop. You can place an order via Labscoop.com, submit your PO by email to firstname.lastname@example.org, by fax at 1.800.316.3081 or by phone at 1.800.316.3081.
Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request.
Q. Do you offer any discounts? A. We conduct an extensive price analysis on each product we release, so you can be confident that you are getting a great price when comparing the quality of our products, the quantity, and S&H charges. For bulk orders (quantities over 5), please submit a bulk quote request and in most cases we will be able to offer some discounts. For quantities under 5, unfortunately we do not offer any additional discounts.
Q. I still have questions, how do I contact technical support? A. No problem! Email us directly at email@example.com
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STORAGE & SAFETY
Recommended to store NADP at -20°C (KEEP FROZEN, DRY, LIGHT SENSITIVE) and all other kit components at 2-8°C (KEEP REFRIGERATED). For lyophilized kit component storage upon reconsistution please refer to the product insert for more information. Avoid repe
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.
12" X 12" X 12"