Quantitative 1-mL UV/Vis spectrophotometric assay detection method, kit sufficient for 100 assays, ready-to-use, quick protocol, ultrahigh accuracy, hypersensitive, reliable, and consistent
The citric acid cycle enzyme aconitase is a member of a growing family of superoxide anion (O2.-) sensitive [4Fe—4S]-containing (de)- hydratases that has been implicated to be important sites of superoxide anion toxicity. The activity of aconitase is sensitive to inactivation by level of O2.- and is modulated by changes in O2.- levels in bacteria and mammalian cells. Thus, inactivation of aconitase can occur in response to oxygen radical generating compounds such as redox cycling agents, higher levels of oxygen or pathological conditions resulting in higher levels of O2.-. The assay is based on conversion of citrate to isocitrate by aconitase, and conversion of isocitrate to α-ketoglutarate by isocitric dehydrogenase. In this process, NADP+ is converted to NADPH, the absorbance of which is measured at 340 nm. The activity of aconitase is calculated as the rate of formation of NADPH and is expressed as milliunits of aconitase/mg protein.
Redoxica's Aconitase Assay Kit has undergone extensive testing with a number of lysate types, has been published in leading scientific journals and after a decade since its market debut has been recognized as an industry leader in Aconitase assaying technologies and other mitochondrial enzyme assay technologies. We developed the assay by utilizing core principles of biochemistry to ensure optimal accuracy, sensitivity, and reproducibility accompanied by a quick and easy-to-use protocol.
|Unit of Measure||Assays|
|Shelf Life||24 MONTHS|
|Made In||United States of America|
|Components||• Aconitase Assay Buffer (2 x 50 mL bottles, Store at 2-8°C)
• Aconitase Lysis Buffer (50 mL bottle, Store at 2-8°C)
• NADP (2 vials, Store at -20°C)
• Isocitric Dehydrogenase (1 vial, Store at: 2-8°C)
|Required Materials Not Included||• Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range with temperature control.
• Quartz semimicro cuvettes capable of holding 1-mL volume.
• Pipettors for accurately measuring various solutions and reagents.
• Dulbecco’s phosphate buffered saline (D-PBS) (Redoxica Product Code: PB9005-500ML)
|Synonyms||Aconitase Assay Kit, Aconitase Activity Assay Kit, Aconitate Hydratase Assay Kit|
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Q. How do I get a lot specific certificate of analysis? A. The latest batch COA is available on the product page of our website. For a prior lot, please submit a request by visiting our "Contact Us" page. Be sure to provide the product code and batch number.
Q. Is there an MSDS available for this product? A. Yes, MSDS is available upon request.
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STORAGE & SAFETY
Recommended to store NADP at -20°C (KEEP FROZEN, DRY, LIGHT SENSITIVE) and all other kit components at 2-8°C (KEEP REFRIGERATED). For lyophilized kit component storage upon reconsistution please refer to the product insert for more information. Avoid repe
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.
12"" X 12"" X 10""