Quantitative 1-mL UV/Vis spectrophotometric detection assay, kit sufficient for 100 assays, quick protocol, highly accurate, sensitive and reliable
The citric acid cycle enzyme aconitase is a member of a growing family of superoxide anion (O2.-) sensitive [4Fe—4S]-containing (de)- hydratases that has been implicated to be important sites of superoxide anion toxicity. The activity of aconitase is sensitive to inactivation by level of O2.- and is modulated by changes in O2.- levels in bacteria and mammalian cells. Thus, inactivation of aconitase can occur in response to oxygen radical generating compounds such as redox cycling agents, higher levels of oxygen or pathological conditions resulting in higher levels of O2.-. The assay is based on conversion of citrate to isocitrate by aconitase, and conversion of isocitrate to α-ketoglutarate by isocitric dehydrogenase. In this process, NADP+ is converted to NADPH, the absorbance of which is measured at 340 nm. The activity of aconitase is calculated as the rate of formation of NADPH and is expressed as milliunits of aconitase/mg protein.
Redoxica's Aconitase Assay Kit has undergone extensive testing with a number of lysate types, has been published in leading scientific journals and after a decade since its market debut has been recognized as an industry leader in Aconitase assaying technologies and other mitochondrial enzyme assay technologies. We developed the assay by utilizing core principles of biochemistry to ensure optimal accuracy, sensitivity, and reproducibility accompanied by a quick and easy-to-use protocol.
|Unit of Measure||Assays|
|Shelf Life||24 MONTHS|
|Made In||United States of America|
|Components||• Aconitase Assay Buffer (2 x 50 mL bottles, Store at 2-8°C)
• Aconitase Lysis Buffer (50 mL bottle, Store at 2-8°C)
• NADP (2 vials, Store at -20°C)
• Isocitric Dehydrogenase (1 vial, Store at: 2-8°C)
|Required Materials Not Included||• Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range with temperature control.
• Quartz semimicro cuvettes capable of holding 1-mL volume.
• Pipettors for accurately measuring various solutions and reagents.
• Dulbecco’s phosphate buffered saline (D-PBS) (Redoxica Product Code: PB9005-500ML)
|Synonyms||Aconitase Assay Kit, Aconitase Activity Assay Kit, Aconitate Hydratase Assay Kit|
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Q. How do I get a lot specific certificate of analysis? A. The latest batch COA is available on the product page of our website. For a prior lot, please submit a request by visiting our "Contact Us" page. Be sure to provide the product code and batch number.
Q. Is there an MSDS available for this product? A. Yes, MSDS is available upon request.
Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request from the product detail page (directly below the order button). You can also submit a quote request from our contact us page.
Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop head quartered in the United States. You can place an order with them by clicking on the "Order on Labscoop" button on the product page or by submitting your purchase order by email to email@example.com, by fax at 1.800.316.3081 or by phone at 1.800.316.3081. You can also place an order with us directly by submitting an "Order Request" from our "Contact Us" page.
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STORAGE & SAFETY
Recommended to store NADP at -20°C (KEEP FROZEN, DRY, LIGHT SENSITIVE) and all other kit components at 2-8°C (KEEP REFRIGERATED). For lyophilized kit component storage upon reconsistution please refer to the product insert for more information. Avoid repe
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.
12"" X 12"" X 10""
Quantitative 1-mL UV/Vis Continous Spectrophotometric Assay, kit sufficient for 100 assays, ready-to-use, quick protocol, ultra high accuracy, hypersensitive, reliable, and consistent