RESEARCH PRODUCTS

Alpha-Ketoglutarate Dehydrogenase (α-KDH) Assay Kit Quantitative 1-mL UV/Vis Continous Spectrophotometric Assay, kit sufficient for 100 assays, ready-to-use, quick protocol, ultra high accuracy, hypersensitive, reliable, and consistent

Ordering information PRODUCT CODE KD-4022K UNIT PRICE $ 649.00 ($6.49 / Assays) AVAILABILITY In Stock  Ready-to-ship On Oct 19, 2018 FULFILLMENT BY LABSCOOP - SHIPS FROM LITTLE ROCK, AR 72201
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Overview

α-Ketoglutarate dehydrogenase complex is a multienzyme complex present in the mitochondria. This enzyme catalyzes the oxidative decarboxylation of α-Ketoglutarate (KG) to succinyl-CoA with concomitant production of NADH which is proportional to the activity of the enzyme. This enzyme has been shown to be inactivated by oxidative stress. Therefore, the activity of this enzyme is a likely target of oxidative inactivation. The assay procedure uses 1-mL assay reaction volume, and requires quartz cuvettes of 1-mL capacity and a spectrophotometer with kinetic function with temperature control. The assay can be performed with isolated mitochondria or whole cell or tissue lysate.

Redoxica's Alpha-Ketoglutarate Dehydrogenase (α-KDH) Assay Kit has undergone extensive testing with a number of lysate types, has been published in leading scientific journals and after a decade since its market debut has been recognized as an industry leader in α-KDH and other mitochondrial enzyme assay technologies. We developed the assay by utilizing core principles of biochemistry to ensure optimal accuracy, sensitivity, and reproducibility accompanied by a quick and easy-to-use protocol.

Specifications

Unit of Measure Assays
Shelf Life 24 MONTHS
Made In United States of America
Components • α-KDH Assay Buffer (2 x 50 mL bottles, Store at -20°C, Toxic)
• α-KDH Lysis Buffer (50 mL bottle, Store at -20°C, Toxic)
• NAD (2 vials, Store at -20°C)
• Coenzyme A (1 mL vial, Store at: -20°C)
• α-KDH Enzyme (1 Unit, Store at -20°C)
Required Materials Not Included • Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range with temperature control.
• Quartz micro cuvettes capable of reading 1 ml reaction volume in a 1-cm light path.
• Cell sonicator or disrupter.
Synonyms Alpha-Ketoglutarate Dehydrogenase Assay Kit, Alpha-Ketoglutarate Dehydrogenase (α-KDH) Assay Kit, α-KDH Assay Kit, Alpha-KDH Assay Kit, 2-Oxoglutarate Dehydrogenase E1 Assay Kit, OGDH Assay Kit, Mitochondrial Enzyme Assay Kit, AKGDH Assay Kit, E1k Assay Kit, OGDC Assay Kit, Oxoglutarate Dehydrogenase Assay Kit

Faq

Q. How much is shipping? A. Free shipping for US customers. If you are located outside of the United States, shipping costs can range between $30 - $250 USD excluding customs/duties/taxes/VAT.

Q. How do I get a lot specific certificate of analysis? A. The latest batch COA is available on the product page of our website. For a prior lot, please submit a request by visiting our "Contact Us" page. Be sure to provide the product code and batch number.

Q. Is there an MSDS available for this product? A. Yes, MSDS is available upon request.

Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request from the product detail page (directly below the order button). You can also submit a quote request from our contact us page.

Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop head quartered in the United States. You can place an order with them by clicking on the "Order on Labscoop" button on the product page or by submitting your purchase order by email to orders@labscoop.com, by fax at 1.800.316.3081 or by phone at 1.800.316.3081. You can also place an order with us directly by submitting an "Order Request" from our "Contact Us" page.

Q. Do you offer any discounts? A. We conduct an extensive price analysis on each product we release, so you can rest assured that you are getting a great price when comparing the quality of our products, the quantity, and free shipping and handling for our US customers. For bulk orders (quantities over 5), please submit a bulk quote request and in most cases we will be able to offer some discounts. For quantities under 5, unfortunately we do not offer any additional discounts.

Q. I still have questions, how do I contact technical support? A. No problem! Email us directly at support@redoxica.com

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STORAGE & SAFETY

Storage Temperature

Recommended to store kit at - 20°C (KEEP FROZEN, DRY). For lyophilized kit component storage upon reconsistution please refer to the product insert for more information. Avoid repeated freeze-thaw cycles as assay accuracy may be affected.

Precautions & Disclaimer

For Laboratory Research and Development Use Only. Not for drug, household or other uses.

SHIPPING & HANDLING

Shipping Conditions

Dry Ice

Hazmat

No

Packaging

Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.

Shipping Weight

6.00 LBS

Shipping Dimensions

12"" X 12"" X 10""

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