Quantitative 1-mL UV/Vis spectrophotometric assay detection method, kit sufficient for 100 assays, ready-to-use, quick protocol, ultra high accuracy, hypersensitive, reliable, and consistent
α-Ketoglutarate dehydrogenase complex is a multienzyme complex present in the mitochondria. This enzyme catalyzes the oxidative decarboxylation of α-Ketoglutarate (KG) to succinyl-CoA with concomitant production of NADH which is proportional to the activity of the enzyme. This enzyme has been shown to be inactivated by oxidative stress. Therefore, the activity of this enzyme is a likely target of oxidative inactivation. The assay procedure uses 1-mL assay reaction volume, and requires quartz cuvettes of 1-mL capacity and a spectrophotometer with kinetic function with temperature control. The assay can be performed with isolated mitochondria or whole cell or tissue lysate.
Redoxica's Alpha-Ketoglutarate Dehydrogenase (α-KDH) Assay Kit has undergone extensive testing with a number of lysate types, has been published in leading scientific journals and after a decade since its market debut has been recognized as an industry leader in α-KDH and other mitochondrial enzyme assay technologies. We developed the assay by utilizing core principles of biochemistry to ensure optimal accuracy, sensitivity, and reproducibility accompanied by a quick and easy-to-use protocol.
|Unit of Measure||Assays|
|Shelf Life||24 MONTHS|
|Made In||United States of America|
|Components||• α-KDH Assay Buffer (2 x 50 mL bottles, Store at -20°C, Toxic)
• α-KDH Lysis Buffer (50 mL bottle, Store at -20°C, Toxic)
• NAD (2 vials, Store at -20°C)
• Coenzyme A (1 mL vial, Store at: -20°C)
• α-KDH Enzyme (1 Unit, Store at -20°C)
|Required Materials Not Included||• Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range with temperature control.
• Quartz micro cuvettes capable of reading 1 ml reaction volume in a 1-cm light path.
• Cell sonicator or disrupter.
|Synonyms||Alpha-Ketoglutarate Dehydrogenase Assay Kit, Alpha-Ketoglutarate Dehydrogenase (α-KDH) Assay Kit, α-KDH Assay Kit, Alpha-KDH Assay Kit, 2-Oxoglutarate Dehydrogenase E1 Assay Kit, OGDH Assay Kit, Mitochondrial Enzyme Assay Kit, AKGDH Assay Kit, E1k Assay Kit, OGDC Assay Kit, Oxoglutarate Dehydrogenase Assay Kit|
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STORAGE & SAFETY
Recommended to store kit at - 20°C (KEEP FROZEN, DRY). For lyophilized kit component storage upon reconsistution please refer to the product insert for more information. Avoid repeated freeze-thaw cycles as assay accuracy may be affected.
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.
12"" X 12"" X 10""
Quantitative 1-mL UV/Vis Continuous Spectrophotometric Assay, kit sufficient for 100 assays, ready-to-use, quick protocol, ultrahigh accuracy, hypersensitive, reliable, and consistent