Achieve new levels of transfection efficiency with Redoxica's DINOFECT™ DNA Transfection Reagent, a potent and highly effective, ready-to-use, non-toxic, high-throughput next generation cationic polymer based transfection reagent optimized for direct application to transfect a broad range of primary cell lines and other cell lines in adherent and in suspension. DINOFECT™ non-liposomal reagent free of animal-derived components, filtered through a 0.2 μm (micron) sterile ensuring low cytotoxic effects resulting in physiologically relevant data and high stability in the presence and absence of serum while also showing higher transfection efficiency in common cell lines when compared to competitors. Key characteristics associated with DNA condensation and endosomal release greatly improves transfection efficiency.
DINOFECT™ Transfection Reagent is multi-component, serum-resistant (exceptionally stable in serum) reagent that forms a complex with DNA allowing for the subsequent transportation of the complex into animal or plant cells. This complex can be added directly into a complete cell medium and assayed for transfection efficiency within 24-72 hrs within the additional hassle of exchanging the media. As a transfection that does not require exchanging media, it is ideal for high-throughput transfection. To prevent cell death and negligible cytotoxicity, the reagent has be designed to be non-toxic allowing for a > 90% cell survival rate when experimentation is carried out as per the accompanying protocol. DINOFECT™ is a cost-effective solution for transient and stable co-transfections and requires only minute amounts of DNA in addition to its suitability for use with the same medium post-transfection. DINOFECT™ may be utilized in a wide range of cell culture and analysis applications.
Since its inception, Redoxica's DINOFECT™ DNA Transfection Reagent is rapidly becoming the gold standard in DNA transfection as it is serves as one of the industry's most reliable, cost-effective, highly scalable transfection reagents accompanied by a quick, simple and consistent protocol that will allow for reproducible transfections in under 30 minutes.
|Unit of Measure||ML|
|Shelf Life||24 MONTHS|
|Sterility||0.2 μm (micron) Sterile Filtered|
|Appearance||Clear, colorless liquid|
|Form||Liquid, aqueous solution|
|Grade||Cell Culture Grade (also suitable for molecular biology applications)|
|Source||Synthetic, Non-Liposomal, Animal Free|
|Components||• 1.5 mL OXFECT™ Transfection Reagent (1 µg/µL)
• 15 µL GFP Plasmid Transfection Control (1 µg/µL)
|Automated Method||Yes, High-Throughput|
|Compatible Cell Lines||Embryonic Stem Cell (Mouse and Human), Postmitotic (Rat), Postmitotic Neurons (Human), P815 (Mouse Matocytoma), NIH3T3 (Mouse Embryonic Fibroblast), BNL CL.2 (Mouse Hepatocytes), C-26 (Mouse Colon Carcinoma Cells), C2C12 (Mouse Myoblasts), CT26 (Mouse Non-immunogenic Colon Carcinoma), L929 (Mouse Subcutaneous Connective Tissue Fibroblasts), MCA-38 (Mouse Colon Carcinoma Cells), Neuro 2A (Mouse Neuroblastoma Cells), LLC-PK1 (Porcine Kidney Epithelial Cells), SH-SY5Y (Human Neuroblastoma Cells), R|
|Transfections||Up to 750|
|Composition||Non-Lipid, Cationic Polymer|
|Suitability||Serum-resistant (stable in presence or absence of serum)|
|Delivery||Directly into Complete Cell Medium (no exchanging of media required)|
|Cell Survival Rate||> 90% when experimentation conducted per provided guidelines|
|Transfection Time||< 30 Minutes|
|Synonyms||DNA Transfection Reagent, DINOFECT™ DNA Transfection Reagent, Embryonic Stem Cell (Mouse and Human) Transfection Reagent, Postmitotic (Rat) Transfection Reagent, Postmitotic Neurons (Human) Transfection Reagent, P815 (Mouse Matocytoma) Transfection Reagent, NIH3T3 (Mouse Embryonic Fibroblast) Transfection Reagent, BNL CL.2 (Mouse Hepatocytes) Transfection Reagent, C-26 (Mouse Colon Carbinoma Cells) Transfection Reagent, C2C12 (Mouse Myoblasts) Transfection Reagent, CT26 (Mouse Non-immunogenic Colon Carcinoma) Transfection Reagent, L929 (Mouse Subcutaneous Connective Tissue Fibroblasts) Transfection Reagent, MCA-38 (Mouse Colon Carbinoma Cells) Transfection Reagent, Neuro 2A (Mouse Neuroblastoma Cells) Transfection Reagent, LLC-PK1 (Porcine Kidney Epithelial Cells) Transfection Reagent, SH-SY5Y (Human Neuroblastoma Cells) Transfection Reagent, RBL2H3 (Rat Basophilic Leukemia) Transfection Reagent, CHO-K1 (Hamster Chinese Ovary Cells, Epithelial) Transfection Reagent, HEK-293 (Human Embryonic Kidney Fibroblast) Transfection Reagent, BOSC 23 (Human Kidney Transformed with Adenovirus 5 DNA) Transfection Reagent, BW5147 (Mouse AKR/JT Cell Lymphoma) Transfection Reagent, B16F10 (Mouse Skin Melanoma Epithelial) Transfection Reagent, Cos-7 (Monkey Kidney Cells) Transfection Reagent, HepG2 (Human Hepatocarcinoma Epithelial) Transfection Reagent, A431 (Human Squamous Carcinoma) Transfection Reagent, SW480 (Human Colon Adenocarcinoma) Transfection Reagent, MCF7 (Human Breast Adenocarcinoma Cells) Transfection Reagent, KB (Human Epithelial Cells), Jurkat (Human T-Cell Leukemia) Transfection Reagent, Hep 2C (Human Epidermal Carcinoma Cells) Transfection Reagent, HeLa (Human Cervix Epitheloid Carcinoma) Transfection Reagent, 56FTH 80 (Human Fetal Trachea Epithellium Cells) Transfection Reagent, 6CFSMEo- (Human Submocosal Gland Epithelium Cells) Transfection Reagent, 9HTEo- (Human Adult Trachea Epithelial Cells) Transfection Reagent, A549 (Human Type II Pneumomcytes) Transfection Reagent, CACO-2 (Human Colorectal Adenocarcinoma Cells) Transfection Reagent, HCS-2/8 (Human Chondrocyte-Like Cells) Transfection Reagent, CFPEo- (Human Trachea Epithelium Cells) Transfection Reagent, CFNPE9o- (Human Nasal Epithelium Cells) Transfection Reagent|
Q. How much is shipping and handling? A. This product is temperature sensitive and does ship on ice. For US customers, S&H charges are $19. For international customers, shipping and handling costs will vary based on location and order size. Additional customs/duties/VAT charges may be incurred for international orders.
Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop. You can place an order via Labscoop.com, submit your PO by email to firstname.lastname@example.org, by fax at 1.800.316.3081 or by phone at 1.800.316.3081.
Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request.
Q. Do you offer any discounts? A. We conduct an extensive price analysis on each product we release, so you can be confident that you are getting a great price when comparing the quality of our products, the quantity, and S&H charges. For bulk orders (quantities over 5), please submit a bulk quote request and in most cases we will be able to offer some discounts. For quantities under 5, unfortunately we do not offer any additional discounts.
Q. I still have questions, how do I contact technical support? A. No problem! Email us directly at email@example.com
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STORAGE & SAFETY
2-8°C (KEEP REFRIGERATED), GFP-Plasmid: -20°C (KEEP FROZEN)
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Borosilicate Glass Vial, Aluminum Canister
5" X 5" X 10"