IPTG (Isopropyl β-D-thiogalactopyranoside) is a non-metabolizable analog of galactose that is commonly used as a molecular biology reagent during cloning procedures that require induction of β-galactosidase activity. IPTG mimics the lactose metabolite allolactose, which triggers transcription of the lac operon by inactivation of the tetrameric lacl repressor by allosterically altering its conformation which prompts the synthesis of β-galactosidase, a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. It is utilized in sync with X-Gal or Bluo-Gal for the purpose of blue-white screening of recombinant bacterial colonies that induce expression of the lac operon in E. coli and also Magenta-Gal for red-white colony screening of bacterial colonies. Allolactose is hydrolyzable by β-galactosidase, but cannot hydrolyze IPTG due to the chemical bond formed with the sulfur atom and as a result it's concentration remains constant during experimentation.
Uptake by E. coli may be independent of lactose permease depending on the concentration of IPTG as there are other transport pathways. At high concentrations which are typically established for protein induction, IPTG enters the cell independent of lactose permease while at low concentrations IPTG enters the cell via lactose permease. Though different concentrations may be utilized depending on the specific use case, IPTG is commonly used with a final concentration ranging from 100 uM - 3 mM. We recommend preparing a stock solution by dissolving IPTG in water and then utilizing a 0.22 um (micron) sterile filter to prepare a 0.1 M IPTG stock solution. The final concentration of IPTG for use in indicator plates should be 0.2 mM. The required induction strength and cell genotype may serve as a key factors in determining the final concentration. IPTG is commonly utilized for its induction efficacy associated with protein expression where a protein of interest is encoded downstream in association with the IPTG and subsequently induced in cell culture which can then be lysed and the resulting protein purified via His-Tag or FST purification systems for proteins with ligand tags. IPTG also has widespread use in blue-white and red-white screening of bacterial colonies.
Redoxica's IPTG is manufactured with high quality materials, the latest instrumentation and manufacturing best practices to ensure an ultrahigh purity end product with reliability and reproducibility at the center of our quality assurance processes. Redoxica's IPTG is manufactured synthetically, from non-animal origin, dioxane-free.
|Unit of Measure||G|
|Shelf Life||60 MONTHS|
|Appearance||White to off-white, powder|
|Form||Solid, fine crystalline powder|
|Grade||Molecular Biology Grade|
|Water||≤ 2.00 %|
|Melting Point (MP)||121°C|
|Purity||≥ 99% (HPLC)|
|Suitability||Suitable for recombinant protein expression and analysis of bacterial induction behavior.|
|Microbial Sterility Test||-1.26|
|5||Dioxane (≤ 0.1%)|
|Synonyms||IPTG, Isopropyl β-D-1-thiogalactopyranoside, Isopropyl β-D-thiogalactoside, 1-Thio-beta-D-galactopyranoside Isopropyl, Isopropyl 1 Thio beta D galactopyranoside, Isopropyl 1-Thio-beta-D-galactopyranoside, Isopropyl Thiogalactoside, Thiogalactoside Isopropyl,|
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STORAGE & SAFETY
Recommended to store IPTG (solid, powder form) dessicated at -20°C (KEEP FROZEN). IPTG may also be dissolved in deionized water, aliquoted and stored for 1-year at -20°C (KEEP FROZEN). Avoid repeated freeze-thaw cycles. IPTG is stable at RT for up to 3 ye
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Borosilicate Glass Bottle
5" X 5" X 10"