RESEARCH PRODUCTS

IPTG (Isopropyl β-D-thiogalactopyranoside), Dioxane-Free ≥ 99% (HPLC), ≤ 0.1% Dioxane, Crystalline Powder, Non-Ionic, Molecular Biology Grade

Ordering information PRODUCT CODE PT9021-50G UNIT PRICE $ 119.00 ($2.38 / G) AVAILABILITY In Stock  Ready-to-ship On Nov 2, 2020 FULFILLMENT BY LABSCOOP - SHIPS FROM LITTLE ROCK, AR 72201
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Overview

IPTG (Isopropyl β-D-thiogalactopyranoside) is a non-metabolizable analog of galactose that is commonly used as a molecular biology reagent during cloning procedures that require induction of β-galactosidase activity. IPTG mimics the lactose metabolite allolactose, which triggers transcription of the lac operon by inactivation of the tetrameric lacl repressor by allosterically altering its conformation which prompts the synthesis of β-galactosidase, a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. It is utilized in sync with X-Gal or Bluo-Gal for the purpose of blue-white screening of recombinant bacterial colonies that induce expression of the lac operon in E. coli and also Magenta-Gal for red-white colony screening of bacterial colonies. Allolactose is hydrolyzable by β-galactosidase, but cannot hydrolyze IPTG due to the chemical bond formed with the sulfur atom and as a result it's concentration remains constant during experimentation.

Uptake by E. coli may be independent of lactose permease depending on the concentration of IPTG as there are other transport pathways. At high concentrations which are typically established for protein induction, IPTG enters the cell independent of lactose permease while at low concentrations IPTG enters the cell via lactose permease. Though different concentrations may be utilized depending on the specific use case, IPTG is commonly used with a final concentration ranging from 100 uM - 3 mM. We recommend preparing a stock solution by dissolving IPTG in water and then utilizing a 0.22 um (micron) sterile filter to prepare a 0.1 M IPTG stock solution. The final concentration of IPTG for use in indicator plates should be 0.2 mM. The required induction strength and cell genotype may serve as a key factors in determining the final concentration. IPTG is commonly utilized for its induction efficacy associated with protein expression where a protein of interest is encoded downstream in association with the IPTG and subsequently induced in cell culture which can then be lysed and the resulting protein purified via His-Tag or FST purification systems for proteins with ligand tags. IPTG also has widespread use in blue-white and red-white screening of bacterial colonies.

Redoxica's IPTG is manufactured with high quality materials, the latest instrumentation and manufacturing best practices to ensure an ultrahigh purity end product with reliability and reproducibility at the center of our quality assurance processes. Redoxica's IPTG is manufactured synthetically, from non-animal origin, dioxane-free.

Specifications

Unit of Measure G
Shelf Life 60 MONTHS
InChI Key BPHPUYQFMNQIOC-NXRLNHOXSA-N
Empirical Formula C9H18O5S
Molecular Weight 238.3
CAS# 367-93-1
Functionality Inducer
Appearance White to off-white, powder
Odor Odorless
Form Solid, fine crystalline powder
Grade Molecular Biology Grade
Water ≤ 2.00 %
Melting Point (MP) 121°C
Source Synthetic, Animal-Free
Purity ≥ 99% (HPLC)
MDL Number MFCD00063273
PubChem CID 656894
Suitability Suitable for recombinant protein expression and analysis of bacterial induction behavior.
EC Number 206-703-0
UNII X73VV2246B
Isomeric SMILES CC(C)S[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O
Canonical SMILES CC(C)SC1C(C(C(C(O1)CO)O)O)O
Formulation 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
Ingredients (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-propan-2-ylsulfanyloxane-3,4,5-triol
Microbial Sterility Test -1.26
0 Yes
1 115 A2
2 AAADceBwOABAAAAAAAAAAAAAAAAAAAAAAAAkAAAAAAAAAAAAAAAAGgQACAAACBSkwAKCAAAABggAAAAAAAAAAAAAABAAAAAAAAABEAIgAAACQAAFAAAjAAHAYAQAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA==
3 Water
4 4631
5 Dioxane (≤ 0.1%)
6 Clear, colorless
Synonyms IPTG, Isopropyl β-D-1-thiogalactopyranoside, Isopropyl β-D-thiogalactoside, 1-Thio-beta-D-galactopyranoside Isopropyl, Isopropyl 1 Thio beta D galactopyranoside, Isopropyl 1-Thio-beta-D-galactopyranoside, Isopropyl Thiogalactoside, Thiogalactoside Isopropyl,

Faq

Q. How much is shipping and handling? A. US customers receive free ground shipping! If you are located outside of the United States, shipping and handling costs will vary based on location and order size. Additional customs/duties/VAT charges may be incurred for international orders.

Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop. You can place an order via Labscoop.com, submit your PO by email to orders@labscoop.com, by fax at 1.800.316.3081 or by phone at 1.800.316.3081.

Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request.

Q. Do you offer any discounts? A. We conduct an extensive price analysis on each product we release, so you can be confident that you are getting a great price when comparing the quality of our products, the quantity, and S&H charges. For bulk orders (quantities over 5), please submit a bulk quote request and in most cases we will be able to offer some discounts. For quantities under 5, unfortunately we do not offer any additional discounts.

Q. I still have questions, how do I contact technical support? A. No problem! Email us directly at support@redoxica.com

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STORAGE & SAFETY

Storage Temperature

Recommended to store IPTG (solid, powder form) dessicated at -20°C (KEEP FROZEN). IPTG may also be dissolved in deionized water, aliquoted and stored for 1-year at -20°C (KEEP FROZEN). Avoid repeated freeze-thaw cycles. IPTG is stable at RT for up to 3 ye

Precautions & Disclaimer

For Laboratory Research and Development Use Only. Not for drug, household or other uses.

SHIPPING & HANDLING

Shipping Conditions

Ambient

Hazmat

No

Packaging

Borosilicate Glass Bottle

Shipping Weight

2.00 LBS

Shipping Dimensions

5" X 5" X 10"