Quantitative 1-mL UV/Vis spectrophotometric assay detection method, kit sufficient for 100 assays, ready-to-use, quick protocol, ultra high accuracy, hypersensitive, reliable, and consistent
NADH dehydrogenase (also known as cytochrome c reductase or NADH oxidoreductase) is another important respiratory chain linked mitochondrial enzyme that catalyzes transdehydrogenations between various pyridine nucleotides. This enzyme is known to be inactivated by oxidative stress. Hydroxyl radicals and superoxide anions were also shown to inactivate this enzyme by 60 and 40% respectively in the mitochondrial fraction. NADH dehydrogenase is measured at 340 nm in the presence of NADH and cytochrome C. The rotenone sensitive dehydrogenase activity (NADH oxidase) is calculated by subtracting rotenone insensitive NADH dehydrogenase activity in presence of rotenone from the total activity measured in the absence of rotenone. The assay procedure uses 1-ml assay reaction volume, and requires quartz cuvettes of 1 ml capacity and a spectrophotometer with kinetic function and equipped with a temperature control capable of maintaining 37°C.
Redoxica's NADH Dehydrogenase Assay Kit has undergone extensive testing with a number of lysate types, has been published in leading scientific journals and after a decade since its market debut has been recognized as the proven, reliable manufacturer of NADH dehydrogenase assay kits. We developed the assay by utilizing core principles of biochemistry to ensure optimal accuracy, sensitivity, and reproducibility accompanied by a quick and easy-to-use protocol.
|Unit of Measure||Assays|
|Shelf Life||24 MONTHS|
|Made In||United States of America|
|Components||• NADH Dehydrogenase Assay Buffer (2 x 50 mL bottles, Store at -20°C)
• NADH Lysis Buffer (50 mL bottle, Store at -20°C)
• NADH (2 vials, Store at -20°C)
• Rotenone (200 uL vial, Store at: -20°C)
• Cytochrome C (1 vial, Store at -20°C, in dark, light sensitive)
|Required Materials Not Included||• Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range at 340 nm, and a temperature control capable of maintaining 37°C).
• Quartz cuvettes capable of reading 1ml reaction volume in a 1-cm light path.
• Cell sonicator or disrupter for cell lysate preparation and a waring blender for tissue sample homogenization.
|Synonyms||NADH Dehydrogenase Assay Kit, NADH Oxidoreductase Assay Kit, Cytochrome C Reductase Assay Kit, Type 1 Dehydrogenase Assay Kit, Type I Dehydrogenase Assay Kit, beta-NADH Dehydrogenase Dinucleotide Assay Kit, β-NADH Dehydrogenase Dinucleotide Assay Kit, βNADH Dehydrogenase Dinucleotide Assay Kit, Diaphorase Assay Kit, Dihydrocodehydrogenase I Dehydrogenase Assay Kit, Dihydrocodehydrogenase 1 Dehydrogenase Assay Kit, Dihydronicotinamide Adenine Dinucleotide Dehydrogenase Assay Kit, Diphosphopyridine Diaphorase Assay Kit, DPNH Diaphorase Assay Kit, NADH Diaphorase Assay Kit, NADH Hydrogenase Assay Kit, NADH-Menadione Oxidoreductase Assay Kit, Reduced Diphosphopyridine Nucleotide Diaphorase Activity Assay Kit, Mitochondrial Enzyme Assay Kit|
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STORAGE & SAFETY
Recommended to store kit at - 20°C (KEEP FROZEN, DRY). For lyophilized kit component storage upon reconsistution please refer to the product insert for more information. Avoid repeated freeze-thaw cycles as assay accuracy may be affected.
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.
12"" X 12"" X 10""
Quantitative 1-mL UV/Vis Continous Spectrophotometric Assay, kit sufficient for 100 assays, ready-to-use, quick protocol, ultra high accuracy, hypersensitive, reliable, and consistent