Researchers at Yale University School of Medicine utilized Redoxica's Thioredoxin Activity Assay technology to measure the Trx2 activity in cardiac mitochondria from human and mouse heart tissue.
Little Rock, AR - United States of America - March 2018
Researchers at Yale University School of Medicine utilized Redoxica's Thioredoxin Activity Assay Kit (Product Code: TR-7011K) to measure the activity of Trx2 in cardiac mitochondria from human and mouse heart tissue lysates. Tissue and cell lysates were quantified for protein concentration by utilizing Bradford assays. The lysates and mitochondria-enriched fractions were used for the assay. The rate of NADPH consumption was measured spectrophotometrically at 340 nm with a spectrophotometer equipped with enzyme kinetics. The Thioredoxin Activity Assay Kit assisted in their work that determined that thioredoxin-2 inhibits mitochondrial reactive oxygen species generation and apoptosis stress kinase-1 activity to maintain cardiac function.