Ultrahigh performance transfection and reproducibility in adherent and suspension cell lines, non-toxic, and serum-compatible
The siRNA is targeted to human thioredoxin and is compatible and recommended to use with Redoxica's DINOFECT™ Transfection Reagent is recommended for transfection. Please follow the DINOFECT™ Transfection Reagent protocol.
siRNAs are small interfering RNA or also sometimes referred to as short interfering RNA or silencing RNA are a class of double-stranded RNA molecules which are among the most abundantly utilized RNA Interference (RNAi) tools for inducing short-term silencing of protein coding genes. They operate within the RNAi pathway and are similar in length to miRNA (20-25 base pairs). Cells that receive transfection of synthetic oligonucleotides well allow for the siRNA to induce gene knockdown. Experimentation for transient silencing, which is possible with siRNA, endure a shorter duration ranging from 2-5 days. siRNA can also be utilized for the knockdown of proteins that are non-coding genes including long noncoding RNAs (lncRNA). siRNAs encompass two strands: an antisense guide strand and a passenger sense strand which forming a duplex of 19 to 25 bp in length. Double stranded siRNAs are often transfected into a cell where the guide strand is loaded into RRISC. This activated protein and nucleic acid complex may then allow for gene silencing by binding to a single target mRNA sequence due to its cleavage and degradation. Polymer based transfection reagents such as Redoxica's DINOFECT™ Transfection Reagent allow for the delivery of siRNA into the cell. Delivery can also occur via electroporation though this may cause in increase in cell death or by addition of chemical modifications to the duplex to assist in delivery though this method may trigger antiviral response in some cells.
|Unit of Measure||nmols|
|Shelf Life||24 MONTHS|
|Concentration||20 µM (20 pmol/µL) (40 µg in a total volume of 150 µL of siRNA dilution buffer)|
|Made In||United States of America|
|Buffer||siRNA dilution buffer 20 mM KCl, 6 mM HEPES-pH 7.5, 0.2 mM MgCl2|
|Synonyms||Thioredoxin siRNA, TRX siRNA, TXN siRNA, TRDX siRNA, TRX1 siRNA, Surface-Associated Sulphydryl Protein siRNA, SASP siRNA, ATL-Derived Factor siRNA, ADF siRNA, TXN Delta siRNA, Thioredoxin Delta siRNA, RP11-427L11.1 siRNA, MGC61975 siRNA, DKFZp686B1993 siRNA|
Q. How much is shipping and handling? A. This product is temperature sensitive and does ship on dry ice. For US customers, S&H charges are $95. For international customers, shipping and handling costs will vary based on location and order size. Additional customs/duties/VAT charges may be incurred for international orders.
Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop. You can place an order via Labscoop.com, submit your PO by email to email@example.com, by fax at 1.800.316.3081 or by phone at 1.800.316.3081.
Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request.
Q. Do you offer any discounts? A. We conduct an extensive price analysis on each product we release, so you can be confident that you are getting a great price when comparing the quality of our products, the quantity, and S&H charges. For bulk orders (quantities over 5), please submit a bulk quote request and in most cases we will be able to offer some discounts. For quantities under 5, unfortunately we do not offer any additional discounts.
Q. I still have questions, how do I contact technical support? A. No problem! Email us directly at firstname.lastname@example.org
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STORAGE & SAFETY
Store at -20°C (KEEP FROZEN, STERILE)
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Borosilicate Glass Bottle, Aluminum Canister
12" X 12" X 12"