Ultra high performance transfection and reproducibility in adherent and suspension cell lines, non-toxic, and serum-compatible
The siRNA is targeted to human thioredoxin and is compatible and recommended to use with Redoxica's DINOFECT™ Transfection Reagent is recommended for transfection. Please follow the DINOFECT™ Transfection Reagent protocol.
siRNAs are small interfering RNA or also sometimes referred to as short interfering RNA or silencing RNA are a class of double-stranded RNA molecules which are among the most abundantly utilized RNA Interference (RNAi) tools for inducing short-term silencing of protein coding genes. They operate within the RNAi pathway and are similar in length to miRNA (20-25 base pairs). Cells that receive transfection of synthetic oligonucleotides well allow for the siRNA to induce gene knockdown. Experimentation for transient silencing, which is possible with siRNA, endure a shorter duration ranging from 2-5 days. siRNA can also be utilized for the knockdown of proteins that are non-coding genes including long noncoding RNAs (lncRNA). siRNAs encompass two strands: an antisense guide strand and a passenger sense strand which forming a duplex of 19 to 25 bp in length. Double stranded siRNAs are often transfected into a cell where the guide strand is loaded into RRISC. This activated protein and nucleic acid complex may then allow for gene silencing by binding to a single target mRNA sequence due to its cleavage and degradation. Polymer based transfection reagents such as Redoxica's DINOFECT™ Transfection Reagent allow for the delivery of siRNA into the cell. Delivery can also occur via electroporation though this may cause in increase in cell death or by addition of chemical modifications to the duplex to assist in delivery though this method may trigger antiviral response in some cells.
|Unit of Measure||nmols|
|Shelf Life||24 MONTHS|
|Concentration||20 µM (20 pmol/µL) (40 µg in a total volume of 150 µL of siRNA dilution buffer)|
|Made In||United States of America|
|Buffer||siRNA dilution buffer 20 mM KCl, 6 mM HEPES-pH 7.5, 0.2 mM MgCl2|
|Synonyms||Thioredoxin siRNA, TRX siRNA, TXN siRNA, TRDX siRNA, TRX1 siRNA, Surface-Associated Sulphydryl Protein siRNA, SASP siRNA, ATL-Derived Factor siRNA, ADF siRNA, TXN Delta siRNA, Thioredoxin Delta siRNA, RP11-427L11.1 siRNA, MGC61975 siRNA, DKFZp686B1993 siRNA|
Q. How much is shipping? A. Free shipping for US customers. If you are located outside of the United States, shipping costs can range between $30 - $250 USD excluding customs/duties/taxes/VAT.
Q. How do I get a lot specific certificate of analysis? A. The latest batch COA is available on the product page of our website. For a prior lot, please submit a request by visiting our "Contact Us" page. Be sure to provide the product code and batch number.
Q. Is there an MSDS available for this product? A. Yes, MSDS is available upon request.
Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request from the product detail page (directly below the order button). You can also submit a quote request from our contact us page.
Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop head quartered in the United States. You can place an order with them by clicking on the "Order on Labscoop" button on the product page or by submitting your purchase order by email to email@example.com, by fax at 1.800.316.3081 or by phone at 1.800.316.3081. You can also place an order with us directly by submitting an "Order Request" from our "Contact Us" page.
Q. Do you offer any discounts? A. We conduct an extensive price analysis on each product we release, so you can rest assured that you are getting a great price when comparing the quality of our products, the quantity, and free shipping and handling for our US customers. For bulk orders (quantities over 5), please submit a bulk quote request and in most cases we will be able to offer some discounts. For quantities under 5, unfortunately we do not offer any additional discounts.
Q. I still have questions, how do I contact technical support? A. No problem! Email us directly at firstname.lastname@example.org
FIND A DISTRIBUTOR
STORAGE & SAFETY
Store at -20°C (KEEP FROZEN, STERILE)
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Borosilicate Glass Bottle, Aluminum Canister
12"" X 12"" X 10""