RESEARCH PRODUCTS

Succinate Dehydrogenase (SDH) Assay Kit Quantitative 1-mL UV/Vis spectrophotometric detection assay, kit sufficient for 100 assays, quick protocol, highly accurate, sensitive and reliable

Ordering information PRODUCT CODE SD-6022K UNIT PRICE $ 598.00 ($5.98 / Assays) AVAILABILITY In Stock  Ready-to-ship On Jun 21, 2018 FULFILLMENT BY LABSCOOP - SHIPS FROM LITTLE ROCK, AR 72201
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Overview

Succinate dehydrogenase is an iron-sulfur cluster protein and is consisted of two sub-units, one 70 kd and the other 27 kd and is embedded in the inner mitochondrial membrane. The electron acceptor of this important mitochondrial enzyme of the TCA cycle is the FAD, which is converted to FADH2 by succinate dehydrogense using two electrons from succinate and forming the fumarate. In the assay we employ K3Fe(CN)6 as the artificial electron acceptor instead of FAD. The rate of decrease in the concentration of K3Fe(CN)6 is monitored at 420 nm at 37°C. A blank reaction rate without succinate is always recorded for specificity. In the assay one mole of succinate reduces 2 moles of K3Fe(CN)6. The rate of reaction is calculated as nmoles of K3Fe(CN)6 reduced per minute at 37°C at pH 7.4 using molar extinction coefficient of K3Fe(CN)6.

Redoxica's Succinate Dehydrogenase (SDH) Assay Kit has undergone extensive testing with a number of lysate types, has been published in leading scientific journals and after a decade since its market debut has been recognized as the reliable manufacturing supplier for SDH assay kits. We developed the assay by utilizing core principles of biochemistry to ensure optimal accuracy, sensitivity, and reproducibility accompanied by a quick and easy-to-use protocol.

Specifications

Unit of Measure Assays
Shelf Life 24 MONTHS
Made In United States of America
Components • SDH Assay Buffer (2 x 50 mL bottles, Store at -20°C, Toxic)
• SDH Lysis Buffer (50 mL bottle, Store at -20°C)
• Sodium Succinate (5mL vial, Store at -20°C)
• Potassium Ferricyanide (1 mL vial, Store at: -20°C, Toxic, Keep Dry)
Required Materials Not Included • Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range in the visible range and a temperature control capable of maintaining 37°C.
• Clear 1-mL cuvettes capable of reading 1-mL reaction volume in a 1-cm light path.
• Cell sonicator or disrupter for cell lysate preparation and a warring blender for tissue sample homogenization.
Synonyms Succinate Dehydrogenase Assay Kit, SDH Assay Kit, Succinate-coenzyme Q Reductase Assay Kit, SQR Assay Kit, Respiratory Complex II Assay Kit, Respiratory Complex 2 Assay Kit, Mitochondrial Enzyme Assay Kit

Faq

Q. How much is shipping? A. Free shipping for US customers. If you are located outside of the United States, shipping costs can range between $30 - $250 USD excluding customs/duties/taxes/VAT.

Q. How do I get a lot specific certificate of analysis? A. The latest batch COA is available on the product page of our website. For a prior lot, please submit a request by visiting our "Contact Us" page. Be sure to provide the product code and batch number.

Q. Is there an MSDS available for this product? A. Yes, MSDS is available upon request.

Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request from the product detail page (directly below the order button). You can also submit a quote request from our contact us page.

Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop head quartered in the United States. You can place an order with them by clicking on the "Order on Labscoop" button on the product page or by submitting your purchase order by email to orders@labscoop.com, by fax at 1.800.316.3081 or by phone at 1.800.316.3081. You can also place an order with us directly by submitting an "Order Request" from our "Contact Us" page.

Q. Do you offer any discounts? A. We conduct an extensive price analysis on each product we release, so you can rest assured that you are getting a great price when comparing the quality of our products, the quantity, and free shipping and handling for our US customers. For bulk orders (quantities over 5), please submit a bulk quote request and in most cases we will be able to offer some discounts. For quantities under 5, unfortunately we do not offer any additional discounts.

Q. I still have questions, how do I contact technical support? A. No problem! Email us directly at support@redoxica.com

Product Citaions

  • Journal of Proteomics

    Jindal, H. K., Merchant, E., Balschi, J. A., Zhangand, Y., & Koren, G. (2012). Proteomic Analyses of Transgenic LQT1 and LQT2 Rabbit Hearts Elucidate an Increase in Expression and Activity of Energy Producing Enzymes. Journal of Proteomics, 75(17), 5254–5265. http://doi.org/10.1016/j.jprot.2012.06.034

    view abstract - Pmid: 22796357

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STORAGE & SAFETY

Storage Temperature

Recommended to store kit at - 20°C (KEEP FROZEN, DRY). For lyophilized kit component storage upon reconsistution please refer to the product insert for more information. Avoid repeated freeze-thaw cycles as assay accuracy may be affected.

Precautions & Disclaimer

For Laboratory Research and Development Use Only. Not for drug, household or other uses.

SHIPPING & HANDLING

Shipping Conditions

Dry Ice

Hazmat

No

Packaging

Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.

Shipping Weight

6.00 LBS

Shipping Dimensions

12"" X 12"" X 10""

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