Quantitative 1-mL UV/Vis spectrophotometric detection assay, kit sufficient for 100 assays, quick protocol, highly accurate, sensitive and reliable
Succinate dehydrogenase is an iron-sulfur cluster protein and is consisted of two sub-units, one 70 kd and the other 27 kd and is embedded in the inner mitochondrial membrane. The electron acceptor of this important mitochondrial enzyme of the TCA cycle is the FAD, which is converted to FADH2 by succinate dehydrogense using two electrons from succinate and forming the fumarate. In the assay we employ K3Fe(CN)6 as the artificial electron acceptor instead of FAD. The rate of decrease in the concentration of K3Fe(CN)6 is monitored at 420 nm at 37°C. A blank reaction rate without succinate is always recorded for specificity. In the assay one mole of succinate reduces 2 moles of K3Fe(CN)6. The rate of reaction is calculated as nmoles of K3Fe(CN)6 reduced per minute at 37°C at pH 7.4 using molar extinction coefficient of K3Fe(CN)6.
Redoxica's Succinate Dehydrogenase (SDH) Assay Kit has undergone extensive testing with a number of lysate types, has been published in leading scientific journals and after a decade since its market debut has been recognized as the reliable manufacturing supplier for SDH assay kits. We developed the assay by utilizing core principles of biochemistry to ensure optimal accuracy, sensitivity, and reproducibility accompanied by a quick and easy-to-use protocol.
|Unit of Measure||Assays|
|Shelf Life||24 MONTHS|
|Made In||United States of America|
|Components||• SDH Assay Buffer (2 x 50 mL bottles, Store at -20°C, Toxic)
• SDH Lysis Buffer (50 mL bottle, Store at -20°C)
• Sodium Succinate (5mL vial, Store at -20°C)
• Potassium Ferricyanide (1 mL vial, Store at: -20°C, Toxic, Keep Dry)
|Required Materials Not Included||• Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range in the visible range and a temperature control capable of maintaining 37°C.
• Clear 1-mL cuvettes capable of reading 1-mL reaction volume in a 1-cm light path.
• Cell sonicator or disrupter for cell lysate preparation and a warring blender for tissue sample homogenization.
|Synonyms||Succinate Dehydrogenase Assay Kit, SDH Assay Kit, Succinate-coenzyme Q Reductase Assay Kit, SQR Assay Kit, Respiratory Complex II Assay Kit, Respiratory Complex 2 Assay Kit, Mitochondrial Enzyme Assay Kit|
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Journal of Proteomics
Jindal, H. K., Merchant, E., Balschi, J. A., Zhangand, Y., & Koren, G. (2012). Proteomic Analyses of Transgenic LQT1 and LQT2 Rabbit Hearts Elucidate an Increase in Expression and Activity of Energy Producing Enzymes. Journal of Proteomics, 75(17), 5254–5265. http://doi.org/10.1016/j.jprot.2012.06.034view abstract - Pmid: 22796357
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STORAGE & SAFETY
Recommended to store kit at - 20°C (KEEP FROZEN, DRY). For lyophilized kit component storage upon reconsistution please refer to the product insert for more information. Avoid repeated freeze-thaw cycles as assay accuracy may be affected.
Precautions & Disclaimer
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
SHIPPING & HANDLING
Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.
12"" X 12"" X 10""