Thioredoxin is a small (12 kDa) ubiquitous protein present in prokaryotes as well as eukaryotes. Thioredoxin maintains the redox pool of the cell, and is known to be induced in conditions of oxidative stress. Thioredoxin is also an anti-oxidative protein that has been shown to provide protection against oxidative stress in cardiovascular diseases, neurodegenerative conditions, and other inflammatory diseases. Increased expression of thioredoxin is known to be associated with increased proliferation of cancer cells. The activity of the thioredoxin system has been reported to be disrupted in various physiological disorders such as HIV infection, Alzheimer disease and neoplastic disease. Therefore, thioredoxin activity is a very useful marker for several diseases. The assay method in the kit is based on the reduction of insulin disulfides by reduced thioredoxin with thioredoxin reductase and NADPH as ultimate electron donor. This assay is simple to use and is optimized to provide a linear rate of reaction.
Thioredoxin is a disulfide reductase which chemically reacts with disulfides converting it to a thiol using NADPH as the source of reducing power. In this process thioredoxin is auto-oxidized. Thioredoxin reductase regenerates the active thiol group of thioredoxin using reducing equivalents from NADPH. The principle of our thioredoxin assay utilizes insulin disulfide as the target for reduction by Trx. One mole of NADPH is required to reduce one mole of insulin disulfide. Therefore, in the assay the rate of disappearance of NADPH is spectrophotometrically measured at 340 nm as a negative slope. The rate of change of absorbance per minute is converted to μmol of NADPH required to reduce insulin disulfide.
The Thioredoxin Activity Assay Kit by Redoxica has undergone extensive testing with a multitude of lysate types, has been published in dozens of leading scientific journals and after a decade since its market debut, remains the undisputed leader in the detection and measurement of Trx activity. We designed the assay by utilizing the core principles of biochemistry to ensure maximum accuracy, sensitivity, and reproducibility accompanied by a quick and easy to use protocol.
| Unit of Measure | Assays |
|---|---|
| Shelf Life | 24 MONTHS |
| Made In | United States of America |
| Components | • Thioredoxin Activity Assay Buffer (2 x 50 mL bottles, Store at 2-8°C) • Insulin (5 mL vial, Store at 2-8°C) • β-NADPH (2 vials, Store at: -20°C, Use within 4-5 hours of reconstitution, Keep reconstituted NADPH on ice) • Thioredoxin (Trx) Positive Control (40 μl tube, Store at -20°C) • Thioredoxin Reductase (TrxR) (300 μl vial; Store at -20°C) • Activation Buffer (250 μl tube; Store at -20°C) |
| Required Materials Not Included | • Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range. • Quartz micro cuvettes capable of reading 50-300 μl volume in a 1-cm light path. |
| Format | Micro-cuvette |
| Synonyms | Thioredoxin Activity Assay Kit, TRX, TXN, TRDX, TRX1, Surface-Associated Sulphydryl Protein, SASP, ATL-Derived Factor, ADF, TXN Delta, Thioredoxin Delta, RP11-427L11.1, MGC61975, DKFZp686B1993 |
Q. How much is shipping? A. Free shipping for US customers. If you are located outside of the United States, shipping costs can range between $30 - $250 USD excluding customs/duties/taxes/VAT.
Q. How do I get a lot specific certificate of analysis? A. The latest batch COA is available on the product page of our website. For a prior lot, please submit a request by visiting our "Contact Us" page. Be sure to provide the product code and batch number.
Q. Is there an MSDS available for this product? A. Yes, MSDS is available upon request.
Q. How do I find pricing and availability? A. The latest pricing and availability is displayed on our website. This is updated in real-time and is the exact same information that our support team and distributors have access to. If you need a formal quotation, please submit a quote request from the product detail page (directly below the order button). You can also submit a quote request from our contact us page.
Q. How do I place an order? A. All Redoxica products are fulfilled by our exclusive global distribution partner, Labscoop head quartered in the United States. You can place an order with them by clicking on the "Order on Labscoop" button on the product page or by submitting your purchase order by email to orders@labscoop.com, by fax at 1.800.316.3081 or by phone at 1.800.316.3081. You can also place an order with us directly by submitting an "Order Request" from our "Contact Us" page.
Q. Do you offer any discounts? A. We conduct an extensive price analysis on each product we release, so you can rest assured that you are getting a great price when comparing the quality of our products, the quantity, and free shipping and handling for our US customers. For bulk orders (quantities over 5), please submit a bulk quote request and in most cases we will be able to offer some discounts. For quantities under 5, unfortunately we do not offer any additional discounts.
Q. I still have questions, how do I contact technical support? A. No problem! Email us directly at support@redoxica.com
Cui, L., Wang, Z., Miao, J., Miao, M., Chandra, R., Jiang, H., … Cui, L. (2012). Mechanisms of in vitro resistance to dihydroartemisinin in Plasmodium falciparum. Molecular Microbiology, 86(1), 111–128. http://doi.org/10.1111/j.1365-2958.2012.08180.x
view abstract - Pmid: 22812578Zieger, M. A., Gupta, M. P., & Wang, M. (2011). Proteomic analysis of endothelial cold-adaptation. BMC Genomics, 12, 630. http://doi.org/10.1186/1471-2164-12-630
view abstract - Pmid: 22192797Huang, Q., Zhou, H. J., Zhang, H., Huang, Y., Hinojosa-Kirschenbaum, F., Fan, P., … Min, W. (2015). Thioredoxin-2 Inhibits Mitochondrial ROS Generation and ASK1 Activity to Maintain Cardiac Function. Circulation, 131(12), 1082–1097. http://doi.org/10.1161/CIRCULATIONAHA.114.012725
view abstract - Pmid: 25628390Recommended to store insulin at 2-8°C (KEEP REFRIGERATED) and all other kit components at - 20°C (KEEP FROZEN, DRY). For lyophilized kit component storage upon reconsistution please refer to the product insert for more information. Avoid repeated freeze-t
For Laboratory Research and Development Use Only. Not for drug, household or other uses.
Dry Ice
No
Each kit utilizes a variety of packaging materials including borosilicate glass, PET, polybags, magnetic board, crosslinked foam, PVC.
6.00 LBS
12"" X 12"" X 10""
Quantitative Assay, suitable for detection of Prx I, II, III, IV, V via Spectrophotometric UV/Vis at 340nm, sufficient for 100 reactions, ultra accurate, ready-to-use, quick protocol, hyper sensitive, high reproducibility
Quantitative Assay, suitable for detection of Prx I, II, III, IV, V via Spectrophotometric UV/Vis at 340nm, sufficient for 100 reactions, ultra accurate, ready-to-use, quick protocol, hyper sensitive, high reproducibility
Quantitative, spectrophotometric, UV/Vis detection, sufficient for 100 reactions, ultra accurate, ready-to-use, quick protocol, hyper sensitive assay with reliable, reproducible results.
